View Our Website View All Jobs

Scientist III (Microbiology)

Scientist Microbiology


• Present 2 research findings/presentations at lab meetings, attend 23 lab meetings; and attend 2 scientific meetings or symposia per year

• Prepare supporting author scientific papers for publication.

• Attend 12 division meetings per year and give 1 presentation per scientist

• Complete 4 quarterly reports and 1 annual progress reports for DTRA per investigator

• Prepare one 1st author manuscript for publication per year

• Prepare supporting author scientific papers for publication.

• Use computer to analyze data, prepare graphs, do statistics, write lab notes and reports.

• Conduct transcriptome analyses to better understand bacterial pathogenesis and design vaccines and therapeutics.

• Create 6 non-BSAT strains for the production of Bacillus anthracis capsule

• Add capsule operon and other control elements in Bacillus anthracis Live Attenuated Vaccine strains performing approximately 18 genetic manipulations per year.

• Perform approximately 3 conjugations per month for strain construction and screen approximately >200 colonies for correct genetic and phenotypic characterizations.

• Perform 6 x 24 PCR reactions for genetic confirmation of strains.

• Create approximately 10 genetic knockouts in Francisella tularensis and Bacillus anthracis for vaccine studies per year

• Make and characterize approximately 48 mutant bacterial strains per year.

• Perform approximately 24 MSD protein assays per year.

• Run approximately 208 PCRs and gel electrophoresis per year.

• Plan and assist in approximately 52 animal experiments per year.

• Analyze approximately 200 animal data sheets per year.

• Prepare approximately 40 DNA/RNA from bacteria and tissues per year.

• Prepare BSL2, BSL3 labs for Safety, Bio-surety and CDC inspections 4 per year

• Run approximately 100 ELISA tests to support anthrax vaccine studies.

• Run approximately 50 ELISAs and other tests [gel electrophoreses] to quantitate and analyze B. anthracis capsule to support anthrax vaccine studies per year.

• Write, plan and execute 2 animal protocols to evaluate experimental anthrax vaccines.

• Assist in 3 animal protocols conducted by other investigators.

• Prepare approximately 20 cultures of B. anthracis for vaccine studies in BSL-3 containment.  

• Prepare spores for animal challenges for analysis of animal immune response to vaccine. 

• Organize approximately 15 airlock decontaminations per year.

• Complete approximately 50 bacterial opsonization assays to evaluate experimental anthrax vaccines.

• Conduct Equipment Inventory Management twice a year in BSL-2 and BSL-3

• Perform monthly Laboratory Management to include safety functions and chemical inventory

Read More

Apply for this position

Apply with
Attach resume as .pdf, .doc, .docx, .odt, or .rtf (limit 5MB) or Paste resume

Paste your resume here or Attach resume file